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Image Search Results
Journal:
Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model
doi: 10.1128/JVI.79.16.10386-10396.2005
Figure Lengend Snippet: Env-specific CD4+ T-cell and CD8+ T-cell responses in 12 macaques. A: Env-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. B: Env-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results are colored as follows: Δ5G SU-specific T cells (red), wt-SU-specific T cells (green), and TM-specific T cells (yellow). Arrows with a dotted line, arrows with broken line, and arrows with a solid line indicate the time of the third DNA vaccination at 8 weeks p.p., the time of the vaccine boost at 21 weeks p.p., and the time of SIVmac239 challenge at 28 weeks p.p., respectively. C: Comparison of SU-specific CD8+ T cells and CD4+ T cells in PBMCs among the wt-Env vaccine group, the Δ5G Env vaccine group, and the vector control group at 26 weeks p.p. and 4, 7, and 12 days p.i. The numbers of SFC responding to SIVmac239 SU were used to compare the effects of the two vaccines. w, weeks; d, days.
Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with
Techniques: Enzyme-linked Immunospot, Comparison, Plasmid Preparation, Vaccines
Journal:
Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model
doi: 10.1128/JVI.79.16.10386-10396.2005
Figure Lengend Snippet: SIV-specific CD8+ T-cell and CD4+ T-cell responses in 12 animals. A: SIV viral-protein-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups: vector controls, wt-Env vaccine group, and Δ5G Env vaccines. B: SIV viral-protein-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results of individual SIV proteins are colored as follows: Gag (red), Nef (green), Tat/Rev (blue), Vif/Vpr/Vpx (yellow), and Pol (pink). C: Comparison of cumulated CD8+ T cells or CD4+ T cells specific to the viral proteins Gag, Pol, Nef, Tat/Rev, and Vif/Vpr/VpX between SIV infection-controlled and uncontrolled animals. w, weeks; d, days.
Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with
Techniques: Enzyme-linked Immunospot, Plasmid Preparation, Vaccines, Comparison, Infection
Journal: Scientific Reports
Article Title: Mitigation of Tacrolimus-Associated Nephrotoxicity by PLGA Nanoparticulate Delivery Following Multiple Dosing to Mice while Maintaining its Immunosuppressive Activity
doi: 10.1038/s41598-020-63767-1
Figure Lengend Snippet: In vivo suppression of T cell proliferation in male mice following a daily subcutaneous injection (for 7 days) of either (1) Normal Saline, (2) Empty PLGA NPs, (3) Prograf, or (4) TAC-loaded PLGA NPs. Flow cytometry analysis was conducted on two cell groups: ( A ) CD4 + T cells and ( B ) CD8 + T cells (n = 6). The percentage of proliferating T cells is presented next to each gate. All samples are composed of the same number of acquired events (10 6 cells).
Article Snippet: Standard surface staining protocol was followed for CD4 + and CD8 + T cells using anti-mouse CD4–APC (R&D systems) and
Techniques: In Vivo, Injection, Saline, Flow Cytometry
Journal: Parasite
Article Title: Expression of TIGIT in splenic and circulatory T cells from mice acutely infected with Toxoplasma gondii
doi: 10.1051/parasite/2021010
Figure Lengend Snippet: Changes in TIGIT expression on T cells in different tissues after T. gondii infection. (A) Proportions of TIGIT + cells among CD4 + and CD8 + T cells in T. gondii- infected (RH) and normal mice (Nc) at 7 days after infection. (B) Results of statistical analysis of the percentage of TIGIT + cells among CD4 + T and CD8 + T cells in RH and Nc mice at 7 days after infection. (C) Dynamic changes in the percentages of TIGIT + in T cells at different time points. The results are representative of three independent experiments with five mice in each group per experiment, with data denoting means ± SDs.
Article Snippet: The antibodies used were as follows: anti-mouse Abs against CD3ε-APC-Vio770, mouse (Miltenyi Biotec, 130-117-676),
Techniques: Expressing, Infection
Journal: Parasite
Article Title: Expression of TIGIT in splenic and circulatory T cells from mice acutely infected with Toxoplasma gondii
doi: 10.1051/parasite/2021010
Figure Lengend Snippet: Changes in CD226 expression on T cells in different tissues after T. gondii infection. (A) Proportions of CD226 + cells among CD4 + T and CD8 + T cells in T. gondii- infected (RH) and normal mice (Nc) at 7 days after infection. (B) Results of statistical analysis of the percentage of CD226 + cells among CD4 + T and CD8 + T in RH and Nc mice at 7 days after infection. (C) Dynamic changes in the percentages of CD226 + T cells at different time points. The results are representative of three independent experiments with five mice in each group per experiment, with data denoting means ± SDs.
Article Snippet: The antibodies used were as follows: anti-mouse Abs against CD3ε-APC-Vio770, mouse (Miltenyi Biotec, 130-117-676),
Techniques: Expressing, Infection